Rapid, Hot Chromotrope Stain for Detecting Microsporidia
نویسندگان
چکیده
منابع مشابه
A rapid stain for Pneumocystis.
Step in procedure Supernatant haemoglobin (g) After thawing 0 45 First wash 1 6 Second wash 0-45 Third wash 0-12 Final product 0-023 After incubation 0-05 stability of the red cell preparation after incubation at 37°C for 30 minutes. Recovery was calculated from the supernatant haemoglobin in relation to the initial total haemo-globin after thawing (table 2). Mean (SD) recovery was 70% (13 3), ...
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By using different staining techniques, 479 stool specimens from 212 diarrheic patients with AIDS were examined for microsporidian spores. Calcofluor fluorescence staining of 119 specimens revealed fluorescent ovoid structures of microsporidian size. Staining of these samples according to the method of Weber et al. (R. Weber, R. T. Bryan, R. L. Owen, C. M. Wilcox, L. Gorelkin, and G. S. Visvesv...
متن کاملHistopathological evaluation of ocular microsporidiosis by different stains
BACKGROUND There is limited data on comparing stains in the detection of microsporidia in corneal biopsies. Hence we wanted to evaluate various stains for their ability to detect microsporidia in corneal tissue sections. METHODS Four cases diagnosed with microsporidiosis on Hematoxylin and Eosin and Periodic Acid Schiff's stained sections of the corneal button between January 2002 and Decembe...
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ژورنال
عنوان ژورنال: Clinical Infectious Diseases
سال: 1999
ISSN: 1058-4838,1537-6591
DOI: 10.1086/520161